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transferrin assay kit #e-80tx  (Immunology Consultants Laboratory Inc)
90
Immunology Consultants Laboratory Inc transferrin assay kit #e-80tx
A – C Levels of total iron, hepcidin, and <t>transferrin</t> in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance.
Transferrin Assay Kit #E 80tx, supplied by Immunology Consultants Laboratory Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taut+and+service/transferrin+assay+kit++e+80tx/pmc09253011-353-0-7
Average 90 stars, based on 1 article reviews
transferrin assay kit #e-80tx - by Bioz Stars, 2026-09
90/100 stars
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1e taux de transfert de l oxygéne était largement suffisant méme dans les pires conditions d opération de l expérience  (New Brunswick Scientific)
86
New Brunswick Scientific 1e taux de transfert de l oxygéne était largement suffisant méme dans les pires conditions d opération de l expérience
A – C Levels of total iron, hepcidin, and <t>transferrin</t> in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance.
1e Taux De Transfert De L Oxygéne était Largement Suffisant Méme Dans Les Pires Conditions D Opération De L Expérience, supplied by New Brunswick Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taut+and+service/1e+conditions+d+dans+de+de+de+exp%C3%A9rience+l+l+largement+les+m%C3%A9me+op%C3%A9ration+oxyg%C3%A9ne+pires+suffisant+taux+transfert+%C3%A9tait/gariepy_stephane__1987__production_de_proteines_alimentaires_par_procede_bacterien_aerobie_thermophile_etude_de_cas_d_un-159-5-22
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1e taux de transfert de l oxygéne était largement suffisant méme dans les pires conditions d opération de l expérience - by Bioz Stars, 2026-09
86/100 stars
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hrp conjugated anti-transferrin  (Immunology Consultants Laboratory Inc)
90
Immunology Consultants Laboratory Inc hrp conjugated anti-transferrin
A – C Levels of total iron, hepcidin, and <t>transferrin</t> in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance.
Hrp Conjugated Anti Transferrin, supplied by Immunology Consultants Laboratory Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taut+and+service/hrp+conjugated+anti+transferrin/pmc03085909-310-42-45
Average 90 stars, based on 1 article reviews
hrp conjugated anti-transferrin - by Bioz Stars, 2026-09
90/100 stars
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elisa kits (rat transferrin and rat ferritin)  (Immunology Consultants Laboratory Inc)
90
Immunology Consultants Laboratory Inc elisa kits (rat transferrin and rat ferritin)
A – C Levels of total iron, hepcidin, and <t>transferrin</t> in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance.
Elisa Kits (Rat Transferrin And Rat Ferritin), supplied by Immunology Consultants Laboratory Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taut+and+service/elisa+kit/pm25205020-247-10-17
Average 90 stars, based on 1 article reviews
elisa kits (rat transferrin and rat ferritin) - by Bioz Stars, 2026-09
90/100 stars
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rat transferrin elisa kit  (Immunology Consultants Laboratory Inc)
90
Immunology Consultants Laboratory Inc rat transferrin elisa kit
A – C Levels of total iron, hepcidin, and <t>transferrin</t> in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance.
Rat Transferrin Elisa Kit, supplied by Immunology Consultants Laboratory Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taut+and+service/rat+transferrin+elisa+kit/pmc04362144-31-11-18
Average 90 stars, based on 1 article reviews
rat transferrin elisa kit - by Bioz Stars, 2026-09
90/100 stars
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bovine transferrin elisa kit  (Immunology Consultants Laboratory Inc)
90
Immunology Consultants Laboratory Inc bovine transferrin elisa kit
A – C Levels of total iron, hepcidin, and <t>transferrin</t> in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance.
Bovine Transferrin Elisa Kit, supplied by Immunology Consultants Laboratory Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taut+and+service/bovine+igg+elisa+kit+e+10g/pm25599608-70-8-12
Average 90 stars, based on 1 article reviews
bovine transferrin elisa kit - by Bioz Stars, 2026-09
90/100 stars
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coloscopie  (DESCENTE Ltd)
90
DESCENTE Ltd coloscopie
A – C Levels of total iron, hepcidin, and <t>transferrin</t> in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance.
Coloscopie, supplied by DESCENTE Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taut+and+service/coloscopie/10__1007_slash_s10190___016___0531___8-197-0-9
Average 90 stars, based on 1 article reviews
coloscopie - by Bioz Stars, 2026-09
90/100 stars
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kit fungitell1  (Associates of Cape Cod Inc)
90
Associates of Cape Cod Inc kit fungitell1
A – C Levels of total iron, hepcidin, and <t>transferrin</t> in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance.
Kit Fungitell1, supplied by Associates of Cape Cod Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taut+and+service/fungitell+assay/pm25498852-29-1-16
Average 90 stars, based on 1 article reviews
kit fungitell1 - by Bioz Stars, 2026-09
90/100 stars
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clip ovesco  (Ovesco Endoscopy USA)
90
Ovesco Endoscopy USA clip ovesco
A – C Levels of total iron, hepcidin, and <t>transferrin</t> in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance.
Clip Ovesco, supplied by Ovesco Endoscopy USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
clip ovesco - by Bioz Stars, 2026-09
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ganglion sentinelle  (Sentinelle Medical)
90
Sentinelle Medical ganglion sentinelle
A – C Levels of total iron, hepcidin, and <t>transferrin</t> in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance.
Ganglion Sentinelle, supplied by Sentinelle Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taut+and+service/ganglion+sentinelle/pm31699399-31-0-18
Average 90 stars, based on 1 article reviews
ganglion sentinelle - by Bioz Stars, 2026-09
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technique du ganglion  (Sentinelle Medical)
90
Sentinelle Medical technique du ganglion
A – C Levels of total iron, hepcidin, and <t>transferrin</t> in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance.
Technique Du Ganglion, supplied by Sentinelle Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
technique du ganglion - by Bioz Stars, 2026-09
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mouse transferrin elisa  (Immunology Consultants Laboratory Inc)
90
Immunology Consultants Laboratory Inc mouse transferrin elisa
A – C Levels of total iron, hepcidin, and <t>transferrin</t> in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance.
Mouse Transferrin Elisa, supplied by Immunology Consultants Laboratory Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taut+and+service/mouse+transferrin+elisa/pm23034013-49-60-63
Average 90 stars, based on 1 article reviews
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Image Search Results


A – C Levels of total iron, hepcidin, and transferrin in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance.

Journal: Cell Death & Disease

Article Title: Iron-overloaded follicular fluid increases the risk of endometriosis-related infertility by triggering granulosa cell ferroptosis and oocyte dysmaturity

doi: 10.1038/s41419-022-05037-8

Figure Lengend Snippet: A – C Levels of total iron, hepcidin, and transferrin in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance.

Article Snippet: Transferrin assay kit (#E-80TX) was obtained from Immunology Consultants Laboratory, Inc.

Techniques: Comparison, Control, Expressing, Fluorescence, Staining